University of Colorado – School of Medicine
Professional Research Assistant
The University of Colorado Denver l Anschutz Medical Campus seeks individuals with demonstrated commitment to creating an inclusive learning and working environment. We value the ability to engage effectively with students, faculty and staff of diverse backgrounds.
*Nature of Work:
The major function of the PRA will be performing molecular biology work including ChIP assay, siRNA work, cell culture work including primary cell lines, ZINK finger technology, RT-PCRs, Seahorse analyses, Western Blotting, and immunohistochemistry, but also to be directly involved in murine studies, including exposure of mice to myocardial-ischemia and acute lung injury induced by mechanical ventilation or LPS/Pseudomonas. Active troubleshooting, team-work and an innovative work approach are expected. In addition, the PRA will also be expected to participate in general laboratory tasks, such as tissue culture work, ordering of supplies etc. Moreover, active participation in the research work, including laboratory meetings of the Eckle laboratories, reading and presenting manuscripts, work in progress presentations, and preparing research manuscripts, grants, animal protocols, or human study protocols are important aspects of the work.
The Eckle laboratory is integrated in the Cardiology Laboratories (Dr. Buttrick/Dr. Walker), the Mucosal Inflammation Program (Dr. Colgan), which represents an interdisciplinary research institute interest in understanding inflammatory diseases and the graduate training program, Department Cell Biology, Stem Cells and Development. Dr. Eckle’s specific research interest is in the development of novel therapeutic approaches towards organ ischemia, including myocardial infarction, and acute lung injury. Key aspects of Dr. Eckle’s research include cardiac/lung metabolism during conditions of low oxygen availability and circadian rhythm protein function in metabolism and different diseases (heart ischemia, lung injury, delirium etc.)
The incumbent will report to Dr. Tobias Eckle, MD, PhD, Professor on a daily basis. The incumbent will receive close supervision by Dr. Eckle’s staff.
After initial training in molecular biology techniques and animal surgery, the PRA will also be involved in the training and supervision of more junior members of the laboratory, including medical students, graduate students or junior professional research assistants and post-docs or research fellows.
_Examples of Work Performed_:
Molecular biology work including conventional reverse transcriptional polymerase chain reaction (RT-PCR) and real-time RT-PCR, protein chemistry, such as immunoprecipitation (IP), Co-IP, chromatin immunoprecipitation (ChIP) assays, generation of permanent knockdown cell lines using ZINK finger or lentivirus technology, and Western blotting; histological techniques such as tissue sections, staining and immunohistochemistry.
Tissue culture work, including feeding and splitting of cultured endothelial, epithelial or myocardial cell lines, work with siRNA silenced cell lines and working with primary cell lines.
Metabolic studies (Seahorse analyses, labeled tracers etc.)
Harvesting and generation of myocardial, endothelial or fibroblast cell lines from neonatal/adult cardiac tissues.
Harvesting and generation of lung epithelial cells from mice.
Assisting with animal work, including myocardial, hepatic, renal ischemia, and ventilator/Pseudomonas/LPS induced lung injury or delirium in mice.
Data gathering and documentation
Assistance with data interpretation and analysis
Presentation of research work and writing manuscripts or grants
_Salary and Benefits:_
Salary is commensurate with skills and experience. The University of Colorado offers a full benefits package. Information on University benefits programs, including eligibility, is located atEmployee Services.
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Your total compensation goes beyond the number on your paycheck. The University of Colorado provides generous leave, health plans and retirement contributions that add to your bottom line.
Total Compensation Calculator:https://www.cu.edu/employee-services/total-compensation
_Diversity and Equity:_
Please click here for information on disability accommodations:http://www.ucdenver.edu/about/departments/HR/jobs/Pages/JobsatCUDenver.aspx
The University of Colorado Denver | Anschutz Medical Campus is committed to recruiting and supporting a diverse student body, faculty and administrative staff. The university strives to promote a culture of inclusiveness, respect, communication and understanding. We encourage applications from women, ethnic minorities, persons with disabilities and all veterans. The University of Colorado is committed to diversity and equality in education and employment.
Bachelor’s degree or equivalent
Excellent written and oral communication skills
Ability to understand and interpret molecular biology terminology
Basic lab bench experience
Experience in using computer-based tools (Word, Outlook, PowerPoint, etc.)
Excellent organization, analytical and interpersonal skills
Organization: *H0001 -- Anschutz Medical Campus
Title: Professional Research Asst
Requisition ID: 15507